LPAisaninertcoprecipitantusedtoaidrecoveryofnucleicacidsduringalcoholprecipitations,essentialforquantitativerecoveryofsmallamountsofnucleicacidsindilutesolutions.LPAoffersseveraladvantagesforrecoveringDNAorstudyingDNA-proteininteractions,relativetoothercarriers,suchastRNAorglycogen.tRNAinterfereswithDNAduringphosphorylationwithpolynucleotidekinaseandglycogencompeteswithproteininDNA-proteininteractionstudies.Incontrast,LPAiscompletelyinert,synthesizedchemically,thereforeisnotcontaminatedwithBIOLOGicalmaterial.ThismakesitidealforuseupstreamofRT-PCR.
LPAhasbeenshowntoprecipitatepicogramamountsofDNAfragmentslargerthan20basepairswhilefailingtoprecipitateshorterfragmentsandfreenucleotides.Linearacrylamideisusefulforseparatingreactionproductsfromunincorporatednucleotidesandfrommostoligonucleotideprimers.ItmaybethemostappropriatecoprecipitanttousewhenprecipitatingDNAandRNAforPCRandRT-PCRreactionssincesmallamountsofcontaminatingnucleicacidspresentinothercarrierscouldbeamplified.LPAshouldbeusedatafinalworkingconcentrationof10-20µg/ml.ItwillnotinterferewithA260/280reADIngs.
| CatalogNo. | : | 10510000-1 | Size | : | 10mg |
