Applications:

Bio-World"sCarboxylCouplingKitconsistsofdiaminodipropylamine(DADPA)Separopore®4B-CLtogenerateafreeprimaryamineattheendofalongspacerarm.Thiskitissuitableforcovalentimmobilizationofpeptidesorothercarboxyl-containing(–COOH)moleculesforuseinaffinitypurificationprocedures.ItincludescarboxylCouplingmatrixaswellasallthenecessarybufferstoperformcouplingreactioninmultiplecolumnuses.Molecules,includingproteins,peptidesandnucleicacids,arecovalentlycoupledtothefreeprimaryamines,andthestablecolumnsareidealforaffinitypurificationofantibodiesandotherinteractingpartners.Moleculescanbecoupledtothefreeaminebynumerousamine-reactivemethods.Theresultingamidebondishighlystableandgreatlyreducesthechanceofleachingoftheaffinitytag.Thelongspacerarmreducessterichindranceandensuresgreaterbindingofproteinsandantibodiesduringaffinitypurification.TheCarboxylCouplingResinhasaveryhighcapacityofimmobilizedDADPAandoffers>60µmoleamineloadforeverymlofresin.Thishighcapacityoffersunsurpassedefficiencyandcapacityintheimmobilizationofcarboxylcontainingproteins.TheCarboxylcouplingkitcanalsobeusedfortheimmobilizationofnucleicacids/oligonucleotidesthrough5’phosphategroups.

KitComponents:

• DADPA-Separopore^®4B-CL(Carboxylcouplingmatrix)2x5mlor5x2ml
• EDCcrosslinker
• Couplingbuffer
• Washbuffer
• Columns/accessories

References:

• Isolationofpolymerasechainreaction-readybacterialDNAfromLakeBaikalsedimentsbycarboxyl-functionalisedmagneticpolymermicrospheres.JChromatogrA.(2006)1130:115-21.

Relatedproducts:

• AminoCouplingKit(SKU#20150000)