bio-World's MagneZoom Magnetic mRNA Isolation Kit is designed to isolate intact poly(A)+ RNA from cells and tissue without requiring phenol or other organic solvents. The technology is based on the coupling of Oligo d(T)₂₅ to 5 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly(A)+ transcripts in the eluent. Intact poly(A)+ RNA which is fully representative of the mRNA population of the original sample can be obtained in less than one hour. Oligo d(T)₂₅ Magnetic Beads can be reused up to three times and the researcher has the option of eluting the isolated mRNA or using the bound dT DNA as a primer in a first-strand cDNA reaction.

Kit Contents, sufficient for 25 reactions:

• Oligo d(T)₂₅ MagneZoom (Magnetic Beads)
• Lysis/Binding Buffer
• Wash Buffers
• Elution Buffer
  Requires MagneZoom paramagnetic bead station (20160000-1) for bead separation.