Norgen's Plasma/Serum Exosome RNA Isolation Kit constitutes an all-in-one system for the isolation and enrichment of exosomal RNA from plasma, serum and ascitic fluid. Purification is based on spin column chromatography using Norgen's proprietary resin as the separation matrix. Briefly, the exosomes are separated from the plasma/serum using Norgen's proprietary resin. Next, the exosomes are lysed to release the RNA, which is then bound to Norgen's resin (BIND). Under these conditions only the RNA will bind to Norgen's resin while the remaining contaminants are removed in the flowthrough or retained on top of the resin. The bound RNA is then washed to remove any remaining impurities (WASH). Lastly, the purified exosome RNA is eluted into 100 µL of the provided Elution Buffer (ELUTE).

The plasma/serum exosomal RNA is isolated free from inhibitors and can be used as template in a number of sensitive downstream assays. The kit is designed to allow for the isolation of exosomal RNA from 50 plasma/serum samples. The kit is designed to allow for the isolation of exosomal RNA from 0.25 mL to 2 mL plasma/serum samples.

FEATURES AND BENEFITS

Fast and easy processing - Rapid spin-column format allows for the processing of multiple samples in under 45 minutes. Versatile Plasma/Serume input volume - Isolate plasma/serum exosomal RNA from 0.25 mL to 2 mL of plasma/serum samples. No phenol-chloroform extractions - Norgen's Plasma/Serum Exosome RNA Isolation Kit isolates RNA without the use of harmful chemicals such as phenol or chloroform. Isolate inhibitor-free RNA suitable for downstream applications - Purified RNA is fully compatible with various downstream applications relating to gene regulation and functional analysis, including RT-PCR, northern blotting and microarray analysis Kit Contents1. PS Solution A2. PS Solution B3. PS Solution C4. Wash Solution5. RNA Elution Solution6. Mini Filter Spin Columns7. Elution Tubes8. Collection Tubes9. Product Insert

Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature (15-25 C) for up to 1 year without showing any reduction in performance.