A sputum specimen is the name given to the mucus which is expectorated from the lower airways. High quality sputum samples should contain very little saliva, as this may contaminate the sputum sample with oral bacteria. Sputum samples are typically evaluated to look for infections such as Moraxella catarrhalis, Mycobacterium tuberculosis, Streptococcus pneumoniae and Haemophilus influenza. Other pathogens can be detected in sputum as well, including HIV. In addition, sputum samples are useful in the detection of lung cancer or to evaluate chronic inflammation.

Purification is based on Norgen's proprietary resin as the separation matrix. Briefly, a sputum sample is collected and a liquefying agent can be added. Next, Binding SolutionI is added, the sample mixed and then centrifuged to concentrate any DNA and cells which may be present. Proteinase K and lysozyme can then be added to aid in the lysis of bacterial cells, followed by the addition of Binding Solution II (BIND) which binds all DNA present within the sample. Under these conditions only the genomic DNA will bind to Norgen's resin, while most of the contaminating RNA and proteinaceous components are removed in the flowthrough following centrifugation. Bound DNA is then washed to remove any remaining impurities (WASH). Lastly the purified genomic DNA is eluted into 100 µL of the provided Elution Buffer (ELUTE).

Norgen's Sputum DNA Isolation Kit constitutes an all-in-one system for the isolation of DNA from sputum samples. The kit allows for the isolation of bacterial or eukaryotic DNA from the sputum samples using a spin column chromatography combined with Norgen's proprietary resin. The genomic DNA is isolated free from inhibitors and can then be used as the template in a number of downstream assays. The kit is designed to allow for the isolation of sputum DNA from 25 samples.

FEATURES AND BENEFITS

• Non-invasive sample collection - Higher donor compliance and lower costs due to non-invasive sample collection • Fast and easy processing - Rapid spin-column format allows for the processing of 10 samples in 30 minutes. • Isolate DNA from small sputum samples - DNA can be isolated and detected from as little as 100 µL of sputum. • High quality DNA is suitable for sensitive downstream assays - Purified DNA is of the highest quality and free from inhibitors, and can be used in sensitive downstream applications including PCR and qPCR • Compatible with Norgen's collection and preservation products - This product allows for the isolation of DNA from samples collected and preserved with Sputum DNA Collection, Preservation and Isolation Kit (Cat#10760130-1).